Mitosis Experimental Design
I. Problem/Question
Will the rate of mitosis in plant cells be affected if the temperature is changed other than ideal temperature.
II. Hypothesis
I believe that making the temperature of the mitosis cycle hotter than the ideal temperature will affect the speed of the mitosis cycle and if I am right, I will see the mitosis phase develop at a slower rate than normal as measured by the time it takes for cells to undergo cytokinesis viewed under a microscope and a time elapsed video over time.
III. Variables
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IV. Experimental GroupsControl Group: A group of plants in normal/ideal temperature.
Experimental Group: Plants that is 25% hotter than ideal temperature. |
V. Experimental Procedure
- Obtain four identical plants. Two for the experimental group and two for the control group. One group of plants will be placed in a greenhouse at ideal temperature and the other set of plants will be put in a different greenhouse, but the temperature will be 25% hotter than the first greenhouse.
- Water the plants every six hours everyday for three weeks.
- Use a camera to record the plant cells developing throughout the day.
- During the weeks, use the microscope to gather a sample of cells daily to record the number of cells at each phase of the cell cycle.
- After the three weeks, get the recorded footage of cell development to observe the rate of cytokinesis.
VI. Materials List
- Microscope
- Camera
- Four identical plants
- Water
- Two identical greenhouses
- Healthy soil
VII. Expected Results
If my hypothesis is correct, I believe the experimental plants will duplicate slower when compared to the control group because the hotter temperature might force the experimental plants to use more energy and not having enough for mitosis.